Improved proteomic analysis following trichloroacetic acid extraction of Bacillus anthracis spore proteins
نویسندگان
چکیده
منابع مشابه
Proteomic analysis of the spore coats of Bacillus subtilis and Bacillus anthracis.
The outermost proteinaceous layer of bacterial spores, called the coat, is critical for spore survival, germination, and, for pathogenic spores, disease. To identify novel spore coat proteins, we have carried out a preliminary proteomic analysis of Bacillus subtilis and Bacillus anthracis spores, using a combination of standard sodium dodecyl sulfate-polyacrylamide gel electrophoresis separatio...
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Differentially expressed and immunogenic spore proteins of the Bacillus cereus group of bacteria, which includes Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis, were identified. Comparative proteomic profiling of their spore proteins distinguished the three species from each other as well as the virulent from the avirulent strains. A total of 458 proteins encoded by 232 open re...
متن کاملMorphogenesis of the Bacillus anthracis spore.
Bacillus spp. and Clostridium spp. form a specialized cell type, called a spore, during a multistep differentiation process that is initiated in response to starvation. Spores are protected by a morphologically complex protein coat. The Bacillus anthracis coat is of particular interest because the spore is the infective particle of anthrax. We determined the roles of several B. anthracis orthol...
متن کاملAnalysis of a novel spore antigen in Bacillus anthracis that contributes to spore opsonization.
The significance of Bacillus anthracis as an agent of bioterrorism has been well established. An understanding of both the pathogenesis and the host response is required to elucidate approaches to more rapidly detect and effectively prevent or treat anthrax. Current vaccine strategies are focused primarily on production of antibodies against the protective antigen components of the anthrax toxi...
متن کاملIdentification of the Immunogenic Spore and Vegetative Proteins of Bacillus anthracis Vaccine Strain A16R
Immunoproteomics was used to screen the immunogenic spore and vegetative proteins of Bacillus anthracis vaccine strain A16R. The spore and vegetative proteins were separated by 2D gel electrophoresis and transferred to polyvinylidene difluoride membranes, and then western blotting was performed with rabbit immune serum against B.anthracis live spores. Immunogenic spots were cut and digested by ...
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ژورنال
عنوان ژورنال: Journal of Microbiological Methods
سال: 2015
ISSN: 0167-7012
DOI: 10.1016/j.mimet.2015.08.008